This page was last edited on 29 august 2018, at 09. Standard antiproliferative chemotherapy is relatively ineffective against slowly proliferating androgenindependent prostate cancer cells within metastatic sites. Thapsia garganica is a very famous medicinal plant known especially for its. Micropropagation of thapsia garganica a medicinal plant a murashige and skoog agar medium supplemented with 0. The mediterranean plant thapsia garganica dicot, apiaceae, also. Effect of solvent polarity on the content of biomolecules and antioxidant activity of thapsia garganica apiaceae for the first time, we have evaluated the antioxidant properties of principal secondary metabolites of methanol, ethyl acetate, hexane and water extracts of thapsia garganica leaves. The antioxidant activity was estimated using 2, 2di phenyl1picrylhydrazyl dpph as free radical scavenger. Thapsia garganica phytotoxicity was studied on lactuca sativa l. It grows to about 70 to 190 cm 28 to 75 in in height. Thapsia garganica firrastrina comune flora italiana. Tg is the most widely employed serca inhibitor michelangeli and east, 2011 and can inhibit serca activity with an ic 50 in the subnanomolar range sagara and inesi, 1991. Tethered lipids are unprecedented in the plant kingdom. Effects of gamma irradiation and comparison of different.
Usda, ars, germplasm resources information network. A taxonomic genus within the family apiaceae deadly carrots. Thapsia garganica, medicinal plant, essential oil, gcms. Thapsia silphion is thought to be identical with thapsia garganica, is found on the mountains near the site of ancient cyrene, and is said to have yielded the gum resin to the ancients as laser cyrenaicum or asa dulces, the greek name being silphion. We developed a two steps procedure coupling accelerated solvent extraction to cpc. A manual of organic materia medica and pharmacognosy. Thapsigargin tg is extracted from the plant thapsia garganica l.
It is used in a poultice as an antitussive treatment of acute bronchitis and pneumonia, in preparations with milk or oil taken orally to treat common lung diseases, and with the direct application of root sections for the soothing of dental pains. Allelopathic effect of the isolated allelochemicals on lactuca sativa l. The two macrocyclic lipids 2hydroxymethyl1,4dioxacycloicosane5,20dione 1 and 3hydroxy1,5dioxacyclohenicosane6,21dione 2 have been isolated from the fruits of thapsia garganica. Five libyan medicinal plants thapsia garganica, hammada scoparia, euphorbia serrata, hyoscya mus albus and retama rateam were selected to evaluate their biological activities. Effects of gamma irradiation and comparison of different extraction. An expeditious, multigram isolation protocol for the. Materials and methods plant materials the aerial parts of ballota pseudodictamnus, salvia fruticosa and thapsia garganica were collected from the aljabal alakdar area, libya, in april 2010. Identification and characterization of a kunzeaol synthase from thapsia garganica. The antioxidant activity extracts was determined using the two free radical. Transcriptome analysis of thapsia laciniata rouy provides. Thapsigargin, a tumor promoter, discharges intracellular. Localization and invivo characterization of thapsia.
Isolation and identification of four bioactive allelochemicals. The present invention relates to a method of producing sesquiterpene lactones of the thapsigargin family, the method comprising the steps of. Thapsigargin is a potent inhibitor of the sarcoendoplasmic reticulum calcium atpase pump, inducing apoptosis in mammalian cells. This is the first time that a 7methoxy4,5dihydro3hoxepin2one ring has been found in a natural product. Isolation of thapsigargin from thapsia garganica l. Thapsigargin amount in different specimens of thapsia garganica l. Ca2930767a1 production of thapsigargins by thapsia cell.
Thapsigargin and nortrilobolide are sesquiterpene lactones found in the mediterranean plant thapsia garganica l. Thapsigargine and thapsigargicine, two new histamine liberators from thapsia garganica l. Theophrastus speaks of its root as emetic and purgative. Show the yellow inflorescence of thapsia garganica, and the mature infructescence of the species. The effects of thapsigargin and siphonodictyal b1 on intracellular calcium levels were not distinguishable in pharmacological experiments conducted with caffeine, ryanodine, muscarine, and thapsigargin in calcium free and calciumcontaining buffer, although thapsigargin was effective at lower concentrations. Thapsia garganica wikispecies, free species directory. Acclimatisation was low52% plantlet survivalas micropropagated plantlets were highly susceptible to. Ali h, christensen sb, foreman jc, pearce fl, piotrowski w, thastrup o. Thapsia, commonly known as the deadly carrots, is a small genus of poisonous plants in the. The plant has been considered specific in treating pain, though caution is advised since it is poisonous to some mammals. Thapsia garganica in the germplasm resources information network grin, u.
Reinvestigation of essential oil content of thapsia garganica grown in the east of algeria pdf. Thapsia garganica thapsia garganica thapsigargin induces proliferation independent apoptosis in prostate cancer cells n hn h o oh c o o h o oh o c o 3c o o c o o h o o o oh o 2n o h o c h psma is an nacetylated alpha psma is a folate hydrolase o naag naa glutamate 24aa extracellular707 aa nh 2 intracellular 19 aa psma. The mediterranean plant thapsia garganica dicot, apiaceae, also known as deadly carrot, produces the highly toxic compound thapsigargin. Seeds, leaves and roots of thapsia garganica as a source of new potent lipases inhibitors. Pmc free article rasmussen u, broogger christensen s, sandberg f. Localization and invivo characterization of thapsia garganica. This book is available for free download in a number of formats including epub, pdf, azw, mobi and more. To comprehend its mode of action on lettuce, seedlings were grown hydroponically in hoagland nutrient solution for two months, then the t. In 1978, the first pharmacological effects of thapsigargin were established and the full structure was elucidated in 1985. They develop into fruits with four wings characteristic of the genus. Plants were identified by dr mohamad abouhadra, faculty. This page was last edited on 29 august 2018, at 08. It has pinnate hairy leaves with sheathlike petioles. Thapsia villosa, commonly known as the villous deadly carrot, is a species of poisonous herbaceous plants in the genus thapsia.
The sesquiterpene lactone thapsigargin is found in the plant thapsia garganica l. Use of a temporary immersion bioreactor system for the. Their center of diversity is around the western mediterranean, extending into the atlantic coasts of portugal and morocco. Thapsia garganica showed high flexibility of functional traits and shifts in the timing of phenological events in response to. Their total phe nolic and flavanoid contents were assessed. Reinvestigation of essential oil content of thapsia garganica grown. Stock cultures of thapsia garganica grown on murashige and skoog agar medium 1962 ms 0. You can also read the full text online using our ereader. All structured data from the file and property namespaces is available under the creative commons cc0 license.
A transcriptomic analysis utilizing illumina nextgeneration sequencing enabled the identification of novel genes involved in the biosynthesis of terpenoids in thapsia. Reinvestigation of essential oil content of thapsia. Thapsia garganica is a very famous medicinal plant known especially for its therapeutic effects such as diuretic, emetic and purgative. An umbelliferous plant, growing in southeastern europe, and well known to the ancients, named from the isle of thapsos, where it was obtained.
Identification and characterization of a kunzeaol synthase. The plant is also strongly rubefacient, producing blisters and intense itching. Their structures were established by physical methods, including xray analysis of transtaganolides a and b. Thapsia garganica allelopathic potentialities explored for.
For centuries preparations containing resin from the root of thapsia garganica l. Pdf essential oil composition of the root bark of thapsia. This mechanism has been used to develop a thapsigarginbased cancer drug first by genspera and later inspyr therapeutics westlake village. The scanned text used to begin the current translation project was obtained from. Effect of solvent polarity on the content of biomolecules. Seeds, leaves and roots of thapsia garganica as a source. Laboratoire des sciences fondamentales, universite amar telidji, bp 37g, laghouat, laboratoire des sciences. A resin is extracted with alcohol from the bark of the root. The flowers are yellow in color and borne on compound umbels. Halima nebeg, khedidja benarous, talia serseg, asma lazreg, hafidha hassani and mohamed yousfi affiliation.
Files are available under licenses specified on their description page. Sesquiterpenoids in thapsia sesquiterpenoids are widespread within the genus thapsia, however, it is thapsigargin and the group of similar compounds, guaianolides, which are of most interest t able 1. Antioxidant and antimicrobial properties of five medicinal. Thapsia, commonly known as the deadly carrots, is a small genus of poisonous plants in the family apiaceae. An ethnopharmacological survey of plants used in traditional diabetes treatment in southeastern algeria ouargla province home.
Pdf micropropagation of thapsia garganicaa medicinal plant. The leaves are dilated at base, onehalf clasping the. Highlights thapsigargin is a very useful tool to study calciumdependent signaling pathways. Four novel and unusual c19 compounds from thapsia transtagana, named transtaganolides a. As a copy of the translation by julius berendes in 1902, it has entered the public domain in the united states and various other countries. Prostatespecific antigenactivated thapsigargin prodrug. Molecules free fulltext thapsigarginfrom thapsia l. Sesquiterpenoids from thapsia species and medicinal. Medium free of pgrs promoted rooting at a low incidence. A very potent and highly selective inhibitor is thapsigargin tg, a sesquiterpene lactone derived from the plant thapsia garganica rasmussen et al. An ethnopharmacological survey of plants used in traditional diabetes treatment in southeastern algeria ouargla province.